| 名稱(chēng) | SRE-Luc/HEK293 |
| 型號(hào) | CBP74030 |
| 報(bào)價(jià) | 50000 |
| 特點(diǎn) | SRE-Luc/HEK293，凍存條件：90% FBS+10% DMSO； |
產(chǎn)品搜索
產(chǎn)品分類(lèi)
相關(guān)文章
- IL4藥物細(xì)胞篩選模型
- 【連載-企業(yè)參考品】肺癌PCR和NGS參考品的選擇
- 【連載-企業(yè)參考品】 急性淋巴細(xì)胞白血病的診斷參考品的選擇
- 細(xì)胞株的緩沖液有哪些用途？
- FGFR2融合靶向藥物細(xì)胞篩選模型
- 細(xì)胞株有關(guān)細(xì)胞培養(yǎng)污染的控制
- AKT E17K突變抑制劑細(xì)胞篩選模型介紹
- 【產(chǎn)品推介】腫瘤融合基因檢測(cè)及靶向治療
- 耐藥ATCC細(xì)胞株如何構(gòu)建看完就會(huì)知道
- 【新品發(fā)布】MRD標(biāo)準(zhǔn)品_40+位點(diǎn)panel標(biāo)準(zhǔn)品
聯(lián)系我們
聯(lián)系人：蔣經(jīng)理
電話(huà)：4008750250
號(hào)碼：
手機(jī)：18066071954
地址：南京市棲霞區(qū)緯地路9號(hào)
Email： zhangxiangwen@cobioer.com
電話(huà)：4008750250
號(hào)碼：
手機(jī)：18066071954
地址：南京市棲霞區(qū)緯地路9號(hào)
Email： zhangxiangwen@cobioer.com
產(chǎn)品展示 / PRODUCTS
藥靶細(xì)胞株 > 腫瘤免疫細(xì)胞株 > CBP74030SRE-Luc/HEK293
- 詳細(xì)內(nèi)容
| CBP74030 | |
| I. Background | |
| The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes. | |
| II. Description | |
| The SRE Reporter -HEK293 cell line is designed for monitoring the activity of the MAPK/ERK signaling pathway. The SRE Reporter -HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line. | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | SRE-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | SRE cell line, MAPK reporter, ERK reporter |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+200ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
| |
Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of Recombinant Human EGF in SRE/Luc HEK293 Cells (C5C16). | |
會(huì)員_a.png)